WANG Min, LI Han, HU Bintao, et al. Anti-inflammatory germacrane-type sesquiterpene lactones from Vernonia sylvatica [J].Chin J Nat Med, 2024, 22(6): 568-576. doi: 10.1016/S1875-5364(24)60656-4
Citation: WANG Min, LI Han, HU Bintao, et al. Anti-inflammatory germacrane-type sesquiterpene lactones from Vernonia sylvatica [J].Chin J Nat Med, 2024, 22(6): 568-576. doi: 10.1016/S1875-5364(24)60656-4

Anti-inflammatory germacrane-type sesquiterpene lactones from Vernonia sylvatica

  • Nine new germacranolides, sylvaticalides A−H (19), and three known analogues (10–12) were isolated from the aerial part of Vernonia sylvatica. Their structures were established using comprehensive spectroscopic analysis, including high-resolution electrospray ionization mass spectroscopy (HR-ESI-MS) and 1D and 2D nuclear magnetic resonance (NMR) spectra. Their absolute configurations were determined by X-ray diffraction experiments. The anti-inflammatory activities of all isolated compounds were assessed by evaluating their inhibitory effects on the nuclear factor kappa B (NF-κB) pathway, which was activated by lipopolysaccharide (LPS)-stimulated human THP1-Dual cells, and the interferon-stimulated gene (ISG) pathway, activated by STING agonist MSA-2 in the same cell model. Compounds 1, 2 and 6 showed inhibitory effects on the NF-κB and ISG signaling pathways, with IC50 values ranging from 4.12 to 10.57 μmol·L−1.
  • loading

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return