Novel sesquiterpenoids isolated from Chimonanthus praecox and their antibacterial activities

In the present study, four new sesquiterpenoids, chimonols A-D (compounds 1 - 4 ), together with four known compounds ( 5 - 8 ) were isolated from the EtOAc extract of Chimonanthus praecox Link. The structures of these new compounds were elucidated on the basis of spectroscopic techniques (UV, IR, MS, and 1D and 2D NMR), and their absolute configurations were established by comparing experimental and calculated electronic circular dichroism (ECD) spectra. Compounds 1 - 8 were evaluated for antimicrobial activities and the minimum inhibitory concentrations (MICs) were determined by the broth microdilution method in 96-well culture plates. Compounds 1 , 2 , and 7 exhibited weak antibacterial effects for S. aureus (ATCC 6538), E. coli (ATCC 11775), and P. aeruginosa (ATCC 10145) with MIC values being 158-249 μg·mL^-1. Compounds 3 - 7 showed activities against C. glabrata (ATCC 2001) and S. aureus (ATCC 43300) with MIC values being 128-197 μg·mL^-1. Compounds 1 - 4 showed activity against S. aureus (ATCC 25923) with MIC values being 162-254 μg·mL^-1. The present study provided a basis for future evaluation of these compounds as antibacterial agents.