Abstract: AIM:To investigate the chemical constituents of the endophytic fungus Verticillium sp.isolated from Rehmannia glutinosa.METHODS:The compounds were isolated and purified by repeated column chromatography,and their structures were determined on the basis of physicochemical properties and spectral analysis.Their cytotoxic and antifungal activities were evaluated.RESULTS:Ten compounds were obtained and their structures were identified as 2,4-dihydroxy-2',6-diacetoxy-3'-methoxy-5'-methyl-diphenyl ether(1),paecilospirone(2),-acetylorcinol(3),2-methoxy-1,8-dimethyl-xanthen-9-one(4),4-hydroxy--lapachone(5),enalin A(6),2,3,4-trimethyl-5,7-dihydroxy-2,3-dihydrobenzofuran(7),4-hydroxyethyl-phenol(8),2,4-dihydroxy-3,5,6-trimethylmethylbenzoate(9),and 3-isopropenyl-(Z)-monomethyl maleate(10).CONCLUSIONS:Compound 1 is a new diphenyl ether,and showed cytotoxic activity against HL-60 cells(IC50 2.24 gmL-1),and antifungal activities against Candida albicans(MIC 8 gmL-1) and Aspergillus fumigatus(MIC 16 gmL-1).