Abstract:
Inthomycins are polyketide antibiotics which contain a terminal carboxamide group and a triene chain. Inthomycin B (
1 ) and its two new analogues
2 and
3 were isolated from the crude extract of
Streptomyces pactum L8. Identification of the gene cluster for inthomycin biosynthesis as well as the
15N-labeled glycine incorporation into inthomycins are described. Combined with the gene deletion of the rare P450 domain in the NRPS module, a formation mechanism of carboxamide moiety in inthomycins was proposed
via an oxidative release of the assembly chain assisted by the P450 domain.
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