Antioxidant activity profiling by spectrophotometric methods of aqueous methanolic extracts of Helichrysum stoechas subsp.rupestre and Phagnalon saxatile subsp.saxatile

Farah Haddouchi; Tarik Mohammed Chaouche; Riadh Ksouri; Faten Medini; Fatima Zohra Sekkal; Abdelhafid Benmansour

Volume 12 Issue 6

Jun. 2014

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Article Navigation > Chinese Journal of Natural Medicines > 2014 > 12(6): 415-422

Citation:

Antioxidant activity profiling by spectrophotometric methods of aqueous methanolic extracts of Helichrysum stoechas subsp.rupestre and Phagnalon saxatile subsp.saxatile

1.
Laboratory of Natural Products, Department of Biology, Faculty of Sciences, Abou Bekr Belkaïd University, B. P 119, Tlemcen, 13000, Algeria;
2.
Laboratory of Plant Adaptation to Abiotic Stresses, Biotechnologic Center in Borj-Cedria Technopol(CBBC), B. P 901, 2050 Hammam-Lif, Tunisia;
3.
Department of Biotechnology, Faculty of Natural Science and Life, Abdelel Hamid Ibn Badiss University, Mostaganem, 27000, Algeria

Corresponding author:
Received Date: 20-Mar.-2013

Abstract

AIM:The aqueous methanolic extracts of two plants from Algeria,Helichrysum stoechas subsp.rupestre and Phagnalon saxatile subsp.saxatile,were investigated for their antioxidant activity.METHOD:Total phenolics,flavonoids,and tannins were determined by spectrophotometric techniques..In vitro antioxidant and radical scavenging profiling was determined by spectrophotometric methods,through:Total antioxidant capacity,and radical scavenging effects by the DPPH and ABTS methods,reducing and chelating power,and blanching inhibition of the -carotene.RESULTS:All of the extracts showed interesting antioxidant and radical scavenging activity.The highest contents in phenolics,tannins,and the highest total antioxidant capacity as gallic acid equivalents of 97.50.33 mg GAE/g DW was obtained for the flowers of H.stoechas subsp.rupestre extract in the phosphomolybdenum assay.An extract of the leafy stems of P.saxatile subsp.saxatile revealed the highest content of flavonoids,and the highest antioxidant activity by the radical scavenging and -carotene assays when compared with standards.The best activity was by the scavenging radical DPPH with an IC50 value of 5.650.10 gmL-1.CONCLUSION:The studied medicinal plants could provide scientific evidence for some traditional uses in the treatment of diseases related to the production of reactive oxygen species(ROS) and oxidative stress.
- Helichrysum stoechas subsp.rupestre,
- Phagnalon saxatile subsp.saxatile,
- Phenolic compounds,
- Antioxidant activity

References

[1]	Velioglu YS,Mazza G,Gao L,et al.Antioxidant activity and total phenolics in selected fruits,vegetables,and grain products[J].J Agric Food Chem,1998,46:4113-4117.
[2]	Aruoma OI.Free radicals,oxidative stress and antioxidants in human health and disease[J].J Am Oil Chem Soc,1998,75(2):199-212.
[3]	Jayaprakasha GK,Rao LJ,Sakariah KK.Antioxidant activities of flavidin in different in vitro model systems[J].Bioorg Med Chem,2004,12:5141-5146.
[4]	Ksouri R,Falleh H,Megdiche W,et al.Antioxidant and antimicrobial activities of the edible medicinal halophyte Tamarix gallica L.and related polyphenolic constituents[J].Food Chem Toxicol,2009,47:2083-2091.
[5]	Pietta PG.Flavonoids as antioxidants[J].J Nat Prod,2000,63:1035-1042.
[6]	Albayrak S,Aksoy A,Sagdic O,et al.Compositions,antioxidant and antimicrobial activities of Helichrysum(Asteraceae) species collected from Turkey[J].Food Chem,2010,119:114-122.
[7]	Lourens ACU,Viljoen AM,Van Herden FR.South African Helichrysum species:a review of the traditional uses,biological activity and phytochemistry[J].J Ethnopharmacol,2008,119:630-652.
[8]	Tutin TG,Heywoo VH,Burgess NA,et al.Flora Europaea:Plantaginaceae to Compositae(and Rubiaceae)[M].Vol 4.Cambridge University Press,1976:133.
[9]	Olmos A,Manez S,Giner RM,et al.Protein tyrosine nitration induced by heme/hydrogen peroxide:inhibitory effect of hydroxycinnamoyl conjugates[J].Planta Med,2007,73(1):20-26.
[10]	Bicchi C,Frattini C,Nano GM,et al.Hexahydrofarnesylacetone from Phagnalon rupestre[J].Relata Tech,1979,11(1):64.
[11]	Dolci M,Tira S.Flavonoids of Gnaphalieae:Phagnalon species[J].Atti Accad Naz Lincei Cl Sci Fis Mat Natur Rend,1982,116:315-318.
[12]	Gngora L,Giner RM,Manez S,et al.New prenylhydroquinone glycosides from Phagnalon rupestre[J].J Nat Prod,2001,64:1111-1113.
[13]	Senatore F,Formisano C,Grassia A,et al.Chemical composition and antibacterial activity of the essential oil of Phagnalon saxatile(L.) Cass.(Asteraceae) growing wild in Southern Italy[J].J Essent Oil Bearing Plants,2005,8:258-263.
[14]	Vermerris W,Nicholson R.Isolation and Identification of Phenolic Compounds.In:Phenolic Compound Biochemistry[M].Springer,New York,2006:152.
[15]	Dewanto VX,Wu K,Adom K,et al.Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity[J].J Agric Food Chem,2002,50:3010-3014.
[16]	Sun B,Richardo-da-Silvia JM,Spranger I.Critical factors of vanillin assay for catechins and proanthocyanidins[J].J Agric Food Chem,1998,46:4267-4274.
[17]	Prieto P,Pineda M,Aguilar M.Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex:specific application to the determination of vitamin E[J].Anal Biochem,1999,269:337-341.
[18]	Atoui AK,Mansouri A,Boskou G,et al.Tea and herbal infusion:Their antioxidant activity and phenolic profile[J].Food Chem,2005,89(1):27-36.
[19]	Oyaizu M.Studies on products of the browning reaction prepared from glucose amine[J].Jpn J Nutr,1986,44:307-315.
[20]	Re,R,Pellegrini N,Proteggente A,et al.Antioxidant activity applying an improved ABTS radical decolorization assay[J].Free Radic Biol Med,1999,26:1231-1237.
[21]	Zhao H,Dong J,Lu J,et al.Effect of extraction solvent mixtures on antioxidant activity evaluation and their extraction capacity and selectivity for free phenolic compounds in barley(Hordeum vulgare L.)[J].J Agric Food Chem,2006,54:7277-7286.
[22]	Koleva Ⅱ,Teris AB,Jozef PH,et al.Screening of plant extracts for antioxidant activity:a comparative study on three testing methods[J].Phytochem Anal,2002,13:8-17.
[23]	Tepe B,Sokmen M,Akpulat AH,et al.In vitro antioxidant activities of the methanol extracts of four Helichrysum species from Turkey[J].Food Chem,2005,90:685-689.
[24]	Vinson JA,Dabbagh YA,Serry MM,et al.Plant flavonoids,especially tea flavonols,are powerful antioxidants using an in vitro oxidation model for heart disease[J].J Agric Food Chem,1995,43:2800-2802.
[25]	Gouveia S,Castilho PC.Helichrysum monizii Lowe:phenolic composition and antioxidant potential[J].Phytochem Anal,2012,23:72-83.
[26]	Dorman HJD,Kosar M,Kahlos K,et al.Antioxidant properties and composition of aqueous extracts from Mentha species,hybrids,varieties and cultivars[J].J Agric Food Chem,2003,51:4563-4569.
[27]	Katalinic V,Mozina SS,Skroza D,et al.Polyphenolic profile,antioxidant properties and antimicrobial activity of grape skin extracts of 14 Vitis vinifera varieties grown in Dalmatia(Croatia)[J].Food Chem,2010,119:715-723.
[28]	Erdogan-Orhan I,Senol FS,Demirci B,et al.Phytochemical characterization of Phagnalon graecum Boiss.by HPLC and GC-MS with its enzyme inhibitory and antioxidant activity profiling by spectrophotometric methods[J].Food Anal Methods,2012:1-9.
[29]	Ozkan G,Sagdic O,Ozcelik H.Some Turkish endemic herb extracts as antimicrobial and antioxidant agents.4th International Congress on Environmental Micropaleontology,Microbiology and Meiobenthology[C].13-18 September,Đsparta,Turkey,2004:151-154.
[30]	Albayrak S,Sagdic O,Aksoy A,et al.Antimicrobial and antioxidant activities of Helichrysum species from the Mediterranean region of Turkey[J].Asian J Chem,2008,20:3143-3152.
[31]	Barros L,Oliveira S,Carvalho AM,et al.In vitro antioxidant properties and characterization in nutrients and phytochemicals of six medicinal plants from the Portuguese folk medicine[J].Ind Crop Prod,2010,32:572-579.
[32]	Barros L,Baptista,P,Correia DM,et al.Effects of conservation treatment and cooking on the chemical composition and antioxidant activity of Portuguese wild edible mushrooms[J].J Agric Food Chem,2007,55:4781-4788.
[33]	Yen GC,Hung CY.Effects of alkaline and heat treatment on antioxidative activity and total phenolics of extracts from Hsian-tsao(Mesona procumbens Hemsl.)[J].Food Res Int,2000,33:487-492.
[34]	Conforti F,Rigano D,Formisano C,et al.Metabolite profile and in vitro activities of Phagnalon saxatile(L.) Cass.relevant to treatment of Alzheimer's disease[J].J Enzym Inhib Med Chem,2010,25(1):97-104.
[35]	Aiyegoro OA,Okoh AI.Preliminary phytochemical screening and in vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC[J].BMC Complem Altern Med,2010,10(1):21.
[36]	Carini M,Aldini G,Furlanetto S,et al.LC coupled to ion trap MS for the rapid screening and detection of polyphenol antioxidants from Helichrysum stoechas[J].Biomed Anal,2001,24:517-526.
[37]	Szge-Seluk S,Birteksz AS.Flavonoids of Helichrysum chasmolycicum and its antioxidant and antimicrobial activities[J].S Afr J Bot,2011,77:170-174.
[38]	Kumaran A,Karunakaran RJ.In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India[J].LWT Food Sci Technol,2007,40:344-352.
[39]	Hernandez I,Alegre L,Van Breusegem F,et al.How relevant are flavonoids as antioxidant in plants[J].Trends Plant Sci,2009,14:125-132.
[40]	Czinner E,Hagymasi K,Blazovics A,et al.In vitro antioxidant properties of Helichrysum arenarium(L.) Moench[J].J Ethnopharmacol,2000,73:437-443.
[41]	Amarowicz R,Wanasundara U,Wanasundara J,et al.Antioxidant activity of ethanolic extracts of flaxseed in a -carotenelinoleate model system[J].J Food Lipids,1993,1:111-117.
[42]	Miraliakbari H,Shahidi F.Antioxidant activity of minor components of tree nut oils[J].Food Chem,2008,111:421-427.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Antioxidant activity profiling by spectrophotometric methods of aqueous methanolic extracts of Helichrysum stoechas subsp.rupestre and Phagnalon saxatile subsp.saxatile

Corresponding author:

1. Laboratory of Natural Products, Department of Biology, Faculty of Sciences, Abou Bekr Belkaïd University, B. P 119, Tlemcen, 13000, Algeria;

2. Laboratory of Plant Adaptation to Abiotic Stresses, Biotechnologic Center in Borj-Cedria Technopol(CBBC), B. P 901, 2050 Hammam-Lif, Tunisia;

3. Department of Biotechnology, Faculty of Natural Science and Life, Abdelel Hamid Ibn Badiss University, Mostaganem, 27000, Algeria

Received Date: 2013-03-20

Abstract: AIM:The aqueous methanolic extracts of two plants from Algeria,Helichrysum stoechas subsp.rupestre and Phagnalon saxatile subsp.saxatile,were investigated for their antioxidant activity.METHOD:Total phenolics,flavonoids,and tannins were determined by spectrophotometric techniques..In vitro antioxidant and radical scavenging profiling was determined by spectrophotometric methods,through:Total antioxidant capacity,and radical scavenging effects by the DPPH and ABTS methods,reducing and chelating power,and blanching inhibition of the -carotene.RESULTS:All of the extracts showed interesting antioxidant and radical scavenging activity.The highest contents in phenolics,tannins,and the highest total antioxidant capacity as gallic acid equivalents of 97.50.33 mg GAE/g DW was obtained for the flowers of H.stoechas subsp.rupestre extract in the phosphomolybdenum assay.An extract of the leafy stems of P.saxatile subsp.saxatile revealed the highest content of flavonoids,and the highest antioxidant activity by the radical scavenging and -carotene assays when compared with standards.The best activity was by the scavenging radical DPPH with an IC50 value of 5.650.10 gmL-1.CONCLUSION:The studied medicinal plants could provide scientific evidence for some traditional uses in the treatment of diseases related to the production of reactive oxygen species(ROS) and oxidative stress.

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Reference (42)

[1]	Velioglu YS,Mazza G,Gao L,et al.Antioxidant activity and total phenolics in selected fruits,vegetables,and grain products[J].J Agric Food Chem,1998,46:4113-4117.
[2]	Aruoma OI.Free radicals,oxidative stress and antioxidants in human health and disease[J].J Am Oil Chem Soc,1998,75(2):199-212.
[3]	Jayaprakasha GK,Rao LJ,Sakariah KK.Antioxidant activities of flavidin in different in vitro model systems[J].Bioorg Med Chem,2004,12:5141-5146.
[4]	Ksouri R,Falleh H,Megdiche W,et al.Antioxidant and antimicrobial activities of the edible medicinal halophyte Tamarix gallica L.and related polyphenolic constituents[J].Food Chem Toxicol,2009,47:2083-2091.
[5]	Pietta PG.Flavonoids as antioxidants[J].J Nat Prod,2000,63:1035-1042.
[6]	Albayrak S,Aksoy A,Sagdic O,et al.Compositions,antioxidant and antimicrobial activities of Helichrysum(Asteraceae) species collected from Turkey[J].Food Chem,2010,119:114-122.
[7]	Lourens ACU,Viljoen AM,Van Herden FR.South African Helichrysum species:a review of the traditional uses,biological activity and phytochemistry[J].J Ethnopharmacol,2008,119:630-652.
[8]	Tutin TG,Heywoo VH,Burgess NA,et al.Flora Europaea:Plantaginaceae to Compositae(and Rubiaceae)[M].Vol 4.Cambridge University Press,1976:133.
[9]	Olmos A,Manez S,Giner RM,et al.Protein tyrosine nitration induced by heme/hydrogen peroxide:inhibitory effect of hydroxycinnamoyl conjugates[J].Planta Med,2007,73(1):20-26.
[10]	Bicchi C,Frattini C,Nano GM,et al.Hexahydrofarnesylacetone from Phagnalon rupestre[J].Relata Tech,1979,11(1):64.
[11]	Dolci M,Tira S.Flavonoids of Gnaphalieae:Phagnalon species[J].Atti Accad Naz Lincei Cl Sci Fis Mat Natur Rend,1982,116:315-318.
[12]	Gngora L,Giner RM,Manez S,et al.New prenylhydroquinone glycosides from Phagnalon rupestre[J].J Nat Prod,2001,64:1111-1113.
[13]	Senatore F,Formisano C,Grassia A,et al.Chemical composition and antibacterial activity of the essential oil of Phagnalon saxatile(L.) Cass.(Asteraceae) growing wild in Southern Italy[J].J Essent Oil Bearing Plants,2005,8:258-263.
[14]	Vermerris W,Nicholson R.Isolation and Identification of Phenolic Compounds.In:Phenolic Compound Biochemistry[M].Springer,New York,2006:152.
[15]	Dewanto VX,Wu K,Adom K,et al.Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity[J].J Agric Food Chem,2002,50:3010-3014.
[16]	Sun B,Richardo-da-Silvia JM,Spranger I.Critical factors of vanillin assay for catechins and proanthocyanidins[J].J Agric Food Chem,1998,46:4267-4274.
[17]	Prieto P,Pineda M,Aguilar M.Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex:specific application to the determination of vitamin E[J].Anal Biochem,1999,269:337-341.
[18]	Atoui AK,Mansouri A,Boskou G,et al.Tea and herbal infusion:Their antioxidant activity and phenolic profile[J].Food Chem,2005,89(1):27-36.
[19]	Oyaizu M.Studies on products of the browning reaction prepared from glucose amine[J].Jpn J Nutr,1986,44:307-315.
[20]	Re,R,Pellegrini N,Proteggente A,et al.Antioxidant activity applying an improved ABTS radical decolorization assay[J].Free Radic Biol Med,1999,26:1231-1237.
[21]	Zhao H,Dong J,Lu J,et al.Effect of extraction solvent mixtures on antioxidant activity evaluation and their extraction capacity and selectivity for free phenolic compounds in barley(Hordeum vulgare L.)[J].J Agric Food Chem,2006,54:7277-7286.
[22]	Koleva Ⅱ,Teris AB,Jozef PH,et al.Screening of plant extracts for antioxidant activity:a comparative study on three testing methods[J].Phytochem Anal,2002,13:8-17.
[23]	Tepe B,Sokmen M,Akpulat AH,et al.In vitro antioxidant activities of the methanol extracts of four Helichrysum species from Turkey[J].Food Chem,2005,90:685-689.
[24]	Vinson JA,Dabbagh YA,Serry MM,et al.Plant flavonoids,especially tea flavonols,are powerful antioxidants using an in vitro oxidation model for heart disease[J].J Agric Food Chem,1995,43:2800-2802.
[25]	Gouveia S,Castilho PC.Helichrysum monizii Lowe:phenolic composition and antioxidant potential[J].Phytochem Anal,2012,23:72-83.
[26]	Dorman HJD,Kosar M,Kahlos K,et al.Antioxidant properties and composition of aqueous extracts from Mentha species,hybrids,varieties and cultivars[J].J Agric Food Chem,2003,51:4563-4569.
[27]	Katalinic V,Mozina SS,Skroza D,et al.Polyphenolic profile,antioxidant properties and antimicrobial activity of grape skin extracts of 14 Vitis vinifera varieties grown in Dalmatia(Croatia)[J].Food Chem,2010,119:715-723.
[28]	Erdogan-Orhan I,Senol FS,Demirci B,et al.Phytochemical characterization of Phagnalon graecum Boiss.by HPLC and GC-MS with its enzyme inhibitory and antioxidant activity profiling by spectrophotometric methods[J].Food Anal Methods,2012:1-9.
[29]	Ozkan G,Sagdic O,Ozcelik H.Some Turkish endemic herb extracts as antimicrobial and antioxidant agents.4th International Congress on Environmental Micropaleontology,Microbiology and Meiobenthology[C].13-18 September,Đsparta,Turkey,2004:151-154.
[30]	Albayrak S,Sagdic O,Aksoy A,et al.Antimicrobial and antioxidant activities of Helichrysum species from the Mediterranean region of Turkey[J].Asian J Chem,2008,20:3143-3152.
[31]	Barros L,Oliveira S,Carvalho AM,et al.In vitro antioxidant properties and characterization in nutrients and phytochemicals of six medicinal plants from the Portuguese folk medicine[J].Ind Crop Prod,2010,32:572-579.
[32]	Barros L,Baptista,P,Correia DM,et al.Effects of conservation treatment and cooking on the chemical composition and antioxidant activity of Portuguese wild edible mushrooms[J].J Agric Food Chem,2007,55:4781-4788.
[33]	Yen GC,Hung CY.Effects of alkaline and heat treatment on antioxidative activity and total phenolics of extracts from Hsian-tsao(Mesona procumbens Hemsl.)[J].Food Res Int,2000,33:487-492.
[34]	Conforti F,Rigano D,Formisano C,et al.Metabolite profile and in vitro activities of Phagnalon saxatile(L.) Cass.relevant to treatment of Alzheimer's disease[J].J Enzym Inhib Med Chem,2010,25(1):97-104.
[35]	Aiyegoro OA,Okoh AI.Preliminary phytochemical screening and in vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC[J].BMC Complem Altern Med,2010,10(1):21.
[36]	Carini M,Aldini G,Furlanetto S,et al.LC coupled to ion trap MS for the rapid screening and detection of polyphenol antioxidants from Helichrysum stoechas[J].Biomed Anal,2001,24:517-526.
[37]	Szge-Seluk S,Birteksz AS.Flavonoids of Helichrysum chasmolycicum and its antioxidant and antimicrobial activities[J].S Afr J Bot,2011,77:170-174.
[38]	Kumaran A,Karunakaran RJ.In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India[J].LWT Food Sci Technol,2007,40:344-352.
[39]	Hernandez I,Alegre L,Van Breusegem F,et al.How relevant are flavonoids as antioxidant in plants[J].Trends Plant Sci,2009,14:125-132.
[40]	Czinner E,Hagymasi K,Blazovics A,et al.In vitro antioxidant properties of Helichrysum arenarium(L.) Moench[J].J Ethnopharmacol,2000,73:437-443.
[41]	Amarowicz R,Wanasundara U,Wanasundara J,et al.Antioxidant activity of ethanolic extracts of flaxseed in a -carotenelinoleate model system[J].J Food Lipids,1993,1:111-117.
[42]	Miraliakbari H,Shahidi F.Antioxidant activity of minor components of tree nut oils[J].Food Chem,2008,111:421-427.

Antioxidant activity profiling by spectrophotometric methods of aqueous methanolic extracts of Helichrysum stoechas subsp.rupestre and Phagnalon saxatile subsp.saxatile

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