Chemical constituents of Euphorbia tibetica and their biological activities

AIM:To investigate the chemical constituents and their biological activities of the aerial parts of Euphorbia tibetica.METHOD:Compounds were isolated and purified by various chromatographic methods,and their structures were elucidated through the use of extensive spectroscopic techniques including 2D-NMR,the structures of compounds 5 and 6 were confirmed by single-crystal X-ray crystallography.Bioactivities of all the isolated compounds were evaluated by the MTT method on A549 and anti-angiogenesis assay.RESULTS:One new compound,methyl 4-epi-shikimate-3-O-gallate(1),together with twenty-three known constituents(2-24) were isolated from the aerial parts of E.tibetica.CONCLUSION:Compound 1 is new,and the other compounds were isolated from this plant for the first time.Compounds 5-7,9,11,17,18 and 20 exhibited inhibitory effects on the growth of human lung cancer cell A549 and compounds 5,7,12,13,17 and 19 showed anti-angiogenic effects in a zebrafish model.