Characterization of thirty-nine polymethoxylated flavonoids(PMFs) in the branches of Murraya paniculata by HPLC-DAD-ESI-MS/MS

AIM:To investigate the polymethoxylated flavonoids(PMFs) in the branches of Murraya paniculata(L.) Jack.METHODS:A sensitive HPLC-DAD-ESI-MS/MS method was established to screen PMFs in the branches of M.paniculata based on the analysis of six PMF standards in the positive mode by CID-MS/MS.RESULTS:The diagnostic fragmentation pathways for polymethoxylated flavones,polymethoxylated flavanones,polymethoxylated chalcones and PMF glycosides were summarized,respectively.According to the MS fragmentation pathways,39 PMFs,including 24 flavones,10 flavanones or chalcones and 5 PMFs glycosides were screened.CONCLUSION:The results indicated that the developed analytical method could be employed as a rapid,effective technique for the chemical screening of PMFs in TCMs extracts.